Alkyl sulfonic acids were analyzed using IC NI-424, an anion analysis column for non-suppressor method. It was shown that the longer alkyl chains result with stronger the hydrophobic interaction between the packing material and the sample. With longer alkyl chains, retention […]
HILICpak VN-50 4D, a polymer-based HILIC mode column, was used to analyze and successfully separate three analytes and their phosphorylated forms. VN-50 4D retains polar substances, which are not retained well by regular reversed-phase mode ODS columns. Thus, it is […]
Ascorbic acid and isoascorbic acid were separated using two columns of RSpak DE-413 (a column for polymer-based reversed phase chromatography). The calibration curve of ascorbic acid and isoascorbic acid shows good linearity respectively in the range from 1 to 100 μg/mL. Sample […]
Furfural and a mix of related acidic and non-acidic impurities are known to inhibit microbial fermentation of lignocellulosic biomass during bio-energy production. In this application, four common furfural and related impurities, a mix of acids and alcohols, were analyzed using […]
Oligogalacturonic acids were separated using HILICpak VN-50 4D, a polymer-based HILIC column. Since galacturonic acid, which is a constituent sugar, has a carboxyl group, it is necessary to keep the eluent under acidic conditions to suppress dissociation. Using a gradient condition of […]
Phytic acid is a phosphoric acid ester of myo-inositol, in which six hydroxyl groups are replaced phosphate group, and it is also called myo-inositol hexaphosphate (IP6). In this application, phytic acid, IP5 (inositol pentaphosphate) and IP4 (inositol tetraphosphate) were simultaneously analyzed […]
Unsaturated fatty acids are expected to prevent arteriosclerosis and thrombosis, and improve visual function and allergy symptoms. In this application, three kinds of Omega-3 fatty acids and two kinds of Omega-6 fatty acids were analyzed using Asahipak ODP-50 4D, a […]
It is believed that Glucuronolactone increases blood flow in the liver, and consequently improves liver detoxification rate. Because of this, it is often included as an ingredient for supplement drinks. The data shows the simultaneous separation of glucuronolactone, organic acids, […]
A column for organic acid analysis, RSpak KC-811 was used to separate D-glucuronic acid and glucuronolactone. Glucuronolacone is a type of lactone, produced by dehydration synthesis of carboxyl group and 3′-hydroxyl group on D-glucuronic acid. Sample : 0.01% each, 20μL […]
Galacturonic acid is one of the uronic acids and is a component of bacterial polysaccharides such as pectin. This chromatogram shows an analysis of galacturonic acid oligomers using an Asahipak NH2P-50 4E column with phosphate buffer as the eluent. […]