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Analysis of Various Oligonucleotides by LC/UV/MS (VN-50 2D)

Feb 9, 20242 min read

Nucleic acids are composed of pentacarbonate, phosphoric acid, and base. While pentacarbonate in RNA is ribose, in DNA, it is deoxyribose (hydroxyl group (OH) at the 2-position of ribose is…

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Analysis of Oligo-DNAs (VN-50 2D)

Feb 9, 20241 min read

In this application, we used a HILICpak VN-50 2D to analyze the unrefined product of synthetic 62-mer oligo-DNA. Under the set condition, oligo-DNAs eluted in the order of smaller to…

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Analysis of 10 – 50mer Oligo-DNAs (VN-50 2D)

Mar 22, 20222 min read

Synthetic oligo-DNAs of 10, 20, 30, 40, and 50mer were analyzed using HILICpak VN-50 2D. Under the given condition, the oligo-DNAs eluted in an order of shorter to longer-chains. Optimization…

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Analysis of 2′-OMe and 2′-MOE Modified Phosphorothioated Oligo-RNA by LC/UV/MS (VN-50 2D)

Mar 17, 20223 min read

See Technical Article: Analysis of Various Oligonucleic Acids With/Without Modifications RNAs are composed of ribose (a pentacarbonate), phosphoric acid, and base. Phosphorothioated oligonucleic acids are often used in nucleic acid…

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