Shodex HILICpak VG-25 2B is a compact semi-micro HILIC column for rapid sugar analysis. In this application, mannose and lactose were separated in less than 6 minutes using isocratic acetonitrile/water conditions, demonstrating fast analysis on the 50 mm column format.
Sample: 2 mg/mL each, 1uL injection
Column: Shodex HILICpak VG-25 2B Eluent: CH₃CN/H₂O = 85/15 Flow rate: 0.2 mL/min Detector: RI Column temperature: 40 °C
Shodex HILICpak VG-25 2B is a semi-micro HILIC column designed for the analysis of sugars and sugar alcohols. The column has a 2.0 mm I.D. × 50 mm format with 2.5 µm polymer-based amino-modified packing material. In this application, fructose, glucose, and sucrose were separated in less than 5 minutes using isocratic acetonitrile/water conditions.
Sample: 2 mg/mL each, 1uL injection
Column: Shodex HILICpak VG-25 2B Eluent: CH₃CN/H₂O = 85/15 Flow rate: 0.2 mL/min Detector: RI Column temperature: 40 °C
Modification of protein is one of research methods of protein function. Functional performance or stability is changed by the modification. PEG modified chymotrypsin is analyzed using PROTEIN KW-802.5.
Sample : 50 μL
PEG-Chymotrypsin 0.1 %
Column : Shodex PROTEIN KW-802.5 (8.0 mm I.D. x 300 mm) Eluent : 10 mM Sodium Phosphate Buffer (pH7.0) Flow rate : 1.0 mL/min Detector : UV (210 nm) Column temp. : 40 °C
In this application, LC/MS/MS analyses of four phosphonic acid amino acid herbicides, including glyphosate and glufosinate, and six of their metabolites were performed using IC SI-50 4D. National Standard of China GB 23200.122-2026*, “National food safety standard – Determination of four pesticides including glyphosate and their metabolites residues in foods of plant origin – Liquid chromatography-tandem mass spectrometry method”, specifies the use of a quaternary ammonium-modified polyvinyl alcohol-based anion-exchange column or a column with equivalent performance.
Note: Although the GB method specifies the use of water for the preparation of N-methylglyphosate stock standard solution, the N-methylglyphosate reagent used in this application was insoluble in water and was therefore dissolved in 1 M NaOH aqueous solution. All other analytical conditions conformed to the GB method.
Preparation of standard solutions
*The version at the time of the application acquisition.
Sample: 10 µL (Sample solution) 1 µg/mL each
Column：Shodex IC SI-50 4D (4.0 mm I.D x 150 mm) Eluent：(A) H2O, (B) 200 mM NH4HCO3 + 30 mM NH3 aq. Gradient; 10 % B (0 to 0.5 min) to 40 % B (1 min) to 80 % B (4 min) to 82.5 % B (5 min) to 95 % B (6 to 12 min) to 10 % B (12.1 to 15 min) Flow rate：0.6 mL/min Detector：ESI-MS (negative, MRM) Column temp.：40 ℃
The Guideline for the Analysis of Prohibited Ingredients in Cosmetics (화장품 중 배합금지성분 분석법 가이드라인), published in Korea in 2024, includes analytical methods for PFAS compounds as well as tetraconazole, a fluorinated pesticide. In this application, six PFAS compounds that are frequently analyzed, together with tetraconazole, were analyzed using UHPLC ODS column C18U 2B. A polymer-based reversed-phase column, Asahipak ODP-50 4B, was used as a delay column* in this analysis method.
*Installing a delay column between the sample injector and the gradient mixer delays the elution of PFAS originating from the LC system to reduce its impact on PFAS quantification.
Sample: 5 µL
(Sample solution) 10 ng/mL each in CH3OH
Column：Shodex C18U 2B (2.0 mm I.D. × 50 mm) Delay column：Shodex Asahipak ODP-50 4B (4.6 mm I.D. × 50 mm) Eluent：(A) 10 mM CH3COONH4 aq., (B) CH3CN (B %) 30 % (0 to 1 min), 30 to 80 % (1 to 11 min), 80 % (11 to 13 min), 80 to 30 % (13 to 14 min), 30 % (14 to 24 min) Flow rate：0.25 mL/min Detector：ESI-MS (MRM) Column temp.：40 ℃
Acetonitrile is commonly used in HILIC applications; however, it is classified as a hazardous substance. In contrast, ethanol is known to have lower toxicity than many other organic solvents. In this application, sugars were analyzed using commercially available 95% ethanol (JIS K 8102 Ethanol (95)) mixture as a mobile phase. Analysis was performed using a polymer-based HILIC column HILICpak VG-50 4D. Sample solutions were prepared by dissolving sugars in an ethanol/water=1/1 (v/v) mixture, followed by 1+9 dilution with ethanol. To account for the higher viscosity of ethanol, the analysis was carried out at an elevated column temperature and a reduced flow rate.
Sample: 10 µL, 1 mg/mL each
Column：Shodex HILICpak VG-50 4D (4.6 mm I.D. × 150 mm) Eluent：95 % Ethanol Flow rate：0.8 mL/min Detector：RI Column temp.：45 ℃
Please use this column with suppressor type ion chromatography system.
Anion standards and organic acids were analyzed using IC SI-50 4D (a column for anions analysis).
Sample: 20 µL
Column：Shodex IC SI-50 4D (4.0 mm I.D x 150 mm) Eluent：3.2 mM Na2CO3 + 1.0 mM NaHCO3 aq. Flow rate：0.7 mL/min Detector：Suppressed conductivity Column temp.：30 ℃
Tetramethylammonium hydroxide (TMAH) is widely used as a photoresist developer in semiconductor manufacturing processes. In photoresist developer solutions, trace levels of cations such as Na+, NH4+, and K+ must be carefully controlled. In this application, standard solutions of TMAH, Na+, NH4+, and K+ were analyzed using a cation chromatography column, IC YS-50. There was a good linearity in the 1 to 100 mg/L range for TMAH.
Sample: 10 µL
Column：Shodex IC YS-50 (4.6 mm I.D. x 125 mm) Eluent：4 mM Methanesulfonic acid aq. Flow rate：1.0 mL/min Detector：Non-suppressed conductivity Column temp.：40 ℃
Tetrahydrofuran (THF) is a widely used mobile phase in organic solvent-based size exclusion chromatography (SEC) due to its excellent solubility for a broad range of polymeric materials. In recent years, growing interest in green chemistry has driven the search for more environmentally friendly organic solvents. 2-Methyltetrahydrofuran (2-MeTHF), which can be produced from renewable resources, and thus offers a lower environmental impact, has attracted attention as a potential alternative to THF. In this application, SEC analyses of polystyrene standards were performed under identical conditions using either 2-MeTHF or THF as the mobile phase, and their separation performance was compared. The results demonstrated that 2-MeTHF provided separation performance comparable to that of THF, confirming its suitability as an alternative mobile phase for organic solvent-based SEC applications.
Sample: 20 µL
Diluted in eluent
Column：Shodex GPC KF-802 (8.0 mm I.D. × 300 mm) Eluent：2-MeTHF, THF Flow rate：1.0 mL/min Detector：UV (254 nm) Column temp.：25 ℃
Using IC YS-50, changing the concentration of methanesulfonic acid in the eluent enables to control cations separation and retention time. Although, retention time of cations will become short if concentration is high, the eluent conductivity (background level) becomes also high.
Sample : 10 μL
1. Li+ 2 ppm
2. Na+ 10 ppm
3. NH4+ 10 ppm
4. K+ 20 ppm
5. Mg2+ 10 ppm
6. Ca2+ 20 ppm
Column : Shodex IC YS-50 (4.6 mm I.D. x 125 mm) Eluent : Methanesulfonic acid aq. Flow rate : 1.0 mL/min Detector : Non-suppressed conductivity Column temp. : 40 °C
