The interrelation between hydrophobicity and retention, and the interrelation between steric selectivity and retention were compared among Shodex columns for reversed phase chromatography. The retention factor (k’) of amylbenzene was used as the retention, the separation factor (α) between n-butylbenzene and n-amino benzene was used as the hydrophobicity. The separation factor between o-terphenil and triphenylene was used as the steric recognition.
Lager separation factor means higher hydrophobicity and higher steric selectivity.

(Interrelation between hydrophobicity and retention)

(Interrelation between steric selectivity and retention)

Column size  : 4.6mmI.D. x 150mm each
Eluent       : H2O/CH3OH=20/80
Flow rate    : 1.0mL/min
Detector     : UV (254nm)
Column temp. : 40°C