Adenosin-5′-monophosphate, adenosin-5′-diphosphate and adenosin-5′-triphosphate were separated using AFpak AAF-894 (an affinity column) with 0.1M Ethylmorpholine-acetate (pH7.0) as the eluent.
Sample : Adenosin phosphate
1. Adenosin-5′-monophosphate, AMP
2. Adenosin-5′-diphosphate, ADP
3. Adenosine-5′-triphosphate, ATP
Column : Shodex AFpak AAF-894 (8.0mmID*50mm)
Eluent : 0.1M Ethylmorpholine-acetate buffer(pH 7.0)
Flow rate : 1.0mL/min
Detector : UV(260nm)
Column temp. : Room temp.
