ODP2 HP does not adsorb protein, because the surface of the packing material is high polar and the pore size is small. ODS columns showed pressure rise as increasing injection times, because BSA is adsorbed packing material. However ODP2 HP showed stable pressure during repeat injections of BSA sample.
Sample : 7.0mg/mL BSA, 5µL
Column : Shodex ODP2 HP-2B (2.0mmID*50mm)
Eluent : 1mM CH3COONH4 aq./CH3CN=90/10
Flow rate : 0.2mL/min
Detector : UV(220nm)
Column temp. : 30°C