The alkali durability test shows that the retention times of each sample
and relative theoretical plate numbers (TPN) using ODP2
HP-4D were virtually unchanged even after 500 hours. On the other hand,
for ODS (A) retention times of
each sample decreased rapidly, reducing TPN after 24 hours, thus showing degradation
of the column.
Test condition of alkali durability:
Eluent : 10mM Phosphate buffer(pH12)/CH3CN=45/55
Flow rate : 0.6mL/min
Column temp. : 30°C
Sample : 5µL
1. Pyridine
200mg/L
2. Phenol
430mg/L 
* TPN before flowing alkali eluent is set as 100% in this
test.
Column : Shodex ODP2 HP-4D (4.6mmID*150mm)
ODS(A) from other manufacturer (4.6mmID*150mm)
Eluent : H2O/CH3OH=70/30
Flow rate : 1.0mL/min
Detector : UV(254nm)
Column temp. : 40°C
