In order to separate hydrophilic compounds, water or 100% buffer solution is used as the eluent, however, such eluent usually shorten the column life of ODS columns. Since the packing material of RSpak DE-413 is chemically stable and has long column life. The stable analysis results can be obtained using DE-413 even when 100% aqueous solution is used as the eluent.
Column : Shodex RSpak DE-413 (4.6mmID*150mm)
Eluent : 10mM H3PO4 aq.
Flow rate : 1.0mL/min
Detector : Shodex RI
Column temp. : 50°C