IIn order to separate hydrophilic compounds, water or 100% buffer solution is used as the eluent, however, such eluent usually shorten the column life of ODS columns. Since the packing material of RSpak
DE-413 is chemically stable and has long column life. The stable analysis results can be obtained using DE-413 even when 100% aqueous solution is used as the eluent.
Sample : Amides, 10micro-L
1. 200mg/mL Acetic acid
2. 20mg/mL Dimethylacetamide
Column : Shodex RSpak DE-413 (4.6mmID*150mm)
Eluent : 10mM Sodium phosphate buffer(pH5.0)
Flow rate : 1.0mL/min
Detector : UV(210nm)
Column temp. : 40°C
