Amino Acids (3) (P-421S)

Amino acids standards were analyzed by CXpak P-421S. Seventeen kinds of amino acids were separated and detected with ninhydrine reaction. The total analytical time was within 50 minutes.

Sample : 0.1 µM each, 100 µL

1. Asp
2. Thr
3. Ser
4. Glu
5. Pro
6. Gly
7. Ala
8. Cystine
9. Val
10. Met
11. Ile
12. Leu
13. Tyr
14. Phe
15. Lys
16. NH₃
17. His
18. Arg

Column       : Shodex CXpak P-421S (4.6 mm I.D. x 150 mm)
Eluent       : MCI BUFFER™ PH Kit (Mitsubishi Chemical Corporation)
               Low pressure step gradient;
               PH‐1 (0 min), PH‐2 (0.2 min), PH‐3 (13.5 min), PH‐4 (23.2 min),
               PH‐RG (47.0 min)
Reagent      : Ninhydrin Coloring Solution Kit for HITACHI
               (FUJIFILM Wako Pure Chemical Corporation)
               R1:R2=50:50
Flow rate    : (Eluent) 0.5 mL/min
               (Reagent) 0.35 mL/min
Detector     : VIS (570 nm)
Column temp. : 63 °C
Reaction temp. : 120 °C