Amino Acids (3) (P-421S)

Amino acid standards are analyzed using CXpak P-421S.  Seventeen kinds of amino acids were separated and detected with the ninhydrine reaction.  The total analytical was within 50 minutes.

Sample : 0.1µM each, 100µL
1. Asp
2. Thr
3. Ser
4. Glu
5. Pro
6. Gly
7. Ala
8. Cys
9. Val
10. Met
11. Ile
12. Leu
13. Tyr
14. Phe
15. Lys
16. NH₃
17. His
18. Arg

Column       : Shodex CXpak P-421S (4.6mmI.D. x 150mm)
Eluent       : MCI Buffer L-8500-PH Kit (Mitsubishi Chemical Corporation)
               Low pressure gradient:
               (0-0.2 min) PH‐1, (0.2-12.5min) PH‐2, (12.5-22.7min) PH‐3,
               (22.7-40.0min) PH‐4, (40.0-53.0min) PH‐RG
Reagent      : Ninhydrin Coloring Solution Kit for HITACHI
               (Wako Pure Chemical Industries, Ltd.)
               (0-52min) R1:R2=50:50
Flow rate    : (Elunt) 0.5mL/min
               (Reagent) 0.35mL/min
Detector     : VIS(570nm)
Column Temp. : 63°C
Reaction Temp. : 120°C