Amino acids were analyzed using IC YS-50 for cations. YS-50 is packed with polyvinyl alcohol with carboxyl functional group. Amino acids are separated by reversed-phase mode and ionic interaction, as NN-814 column does Alanine and Glycine elute at the same elution time under this condition. See also Amino Acids (2) (NN-814) .
Sample: 0.02% each, 5μL
1. Aspartic acid
2. Glycine
3. Valine
4. Methionine
5. Isoleucine
Column : Shodex IC YS-50 (4.6mmI.D. x 125mm) Eluent : 2mM Acetic acid aq. Flow rate : 1.0mL/min Detector : Shodex RI Column temp. : 40°C
