Anticonvulsants in serum were analyzed by a polymer-based reversed phase column, ODP2 HP-4E. Surface of packed gel in ODP2 HP has higher polarity than gels in regular RP columns. Also, its small pore size helps to exclude proteins. Therefore, only a simple sample pretreatment is required to analyze anticonvulsants in serum without the interference from proteins.
Sample pretreatment:
Mix the same volumes of serum and acetonitrile.
Centrifuge the mixture at 6000 g for 5 minutes.
Use the supernatant as sample.
Sample : 20μL
(1) 2-Acetaminophenol 10μg/mL (I.S.)
(2) Zonisamide 13.0μg/mL
(3) Phenobarbital 19.0μg/mL
(4) Carbamazepine 4.5μg/mL
(5) Phenytoin 9.0μg/mL
Column : Shodex ODP2 HP-4E (4.6mmI.D. x 250mm) Eluent : 25mM Sodium phosphate buffer(pH5.2)/CH3CN=680/320 Flow rate : 0.35mL/min Detector : UV(210nm) Column temp. : 40°C
Data courtesy of Katsuko Hara.MT Yutaka Komiyama.PhD,
Department of Clinical Sciences and Laboratory Medicine, Kansai Medical University.
