Carboplatin, a type of anticancer drug, is called a platinum agent because it contains platinum in its structure. A silica-based amino column is used in Assay of Carboplatin listed in the United States Pharmacopeia and the National Formulary (USPNF 2022 Issue 1*). With reference to this USP monograph, carboplatin was analyzed using Asahiak NH2P-50 4E, a polymer-based amino column. We confirmed that NH2P-50 4E was also suitable for the analysis.
*The version at the time of the application acquisition.
Sample: 10 µL
1.Carboplatin 1 mg/mL
Column：Shodex Asahipak NH2P-50 4E (4.6 mm I.D. x 250 mm) Eluent：CH3CN/H2O=83/17 Flow rate：1.5 mL/min Detector：UV (230 nm) Column temp.：25 ℃