Analysis of Choline and Acetylcholine (YS-50)

Choline and acetylcholine were analyzed using IC YS-50, a cation analysis column, under cation exchange mode. If high sensitive analysis is required, LC/MS/MS analysis using HILICpak VC-50 2D, a polymer-based HILIC mode column, is recommended. When VC-50 2D is used, the elution order of a sample is reversed when compared to HILIC mode.
Refer to: LC/MS Analysis of Choline and Acetylcholine (VC-50 2D)

Sample : 10µg/mL each (in H₂O), 20µL
1. Choline
2. Acetylcholine

Column       : Shodex IC YS-50 (4.6mmI.D. x 125mm)
Eluent       : 10mM H3PO4 aq.
Flow rate    : 1.0mL/min
Detector     : Non-suppressed conductivity, UV(210nm)
Column temp. : 40°C