Ethyl α-D-glucoside is an umami component specifically contained in sake. It was found that this component has moisturizing function, and has been drawing attention in recent years. In this application, ethyl α-D-glucoside in sake was analyzed using HILICpak VG-50 4E, a polymer-based amino column. The mixed solvent of ethyl α-D-glucoside and some saccharides were used as the standard solution. The calibration curves using standard solution show good linearity respectively in the range from 0.05 to 1.0%. (Each coefficient of determination (R2) is 0.9999 or more)
Sample : 10μL
Sake (Japanese rice wine) (A)
Sake (Japanese rice wine) (B)
1. Ethyl α-D-glucoside
3. Glucose
Sample : 10μL
Standard solution 0.5% each
1. Ethyl α-D-glucoside
2. Fructose
3. Glucose
4. Sucrose
5. Maltose
Sample : 10μL
Column : Shodex HILICpak VG-50 4E (4.6mmI.D. x 250mm) Eluent : H2O/CH3CN=20/80 Flow rate : 1.0mL/min Detector : Shodex RI Column temp. : 40°C
