Components of fluctooligosugar were separated using two sugar columns in series, SUGAR
KS-802 and
KS-801 .
Only mobile phase of water was required for this separation.
Sample : : 20µL, 0.1% each
1. 1-Fructofuranosyl-D-nystose
2. Nystose
3. 1-Kestose
4. Sucrose
5. Glucose
6. Fructose
Column : Shodex SUGAR KS-802 + KS-801 (8.0mmID*300mm each)
Eluent : H2O
Flow rate : 0.5mL/min
Detector : Shodex RI
Column temp. : 85° C
