A column for organic acid analysis, RSpak KC-811 was used to separate D-glucuronic acid and glucuronolactone. Glucuronolacone is a type of lactone, produced by dehydration synthesis of carboxyl group and 3′-hydroxyl group on D-glucuronic acid.
Sample : 0.01% each, 20μL
1. D-Glucronic acid
Column : Shodex RSpak KC-811 (8.0mmI.D. x 300mm) Eluent : 3mM HClO4 aq. Flow rate : 1.0mL/min Detector : Shodex RI Column temp. : 40°C