Asahipak ODP-50 4D, a reversed phase chromatography column, was used for simultaneous analysis of erythromycin, a macrolide antibiotics, and its derivative azithromycin. Since macrolide antibiotics are basic substances, their dissociation is suppressed under alkaline conditions, and this makes them easily retained in reversed phase analysis. ODP-50 4D has an excellent durability towards alkaline conditions, and thus enables this analysis.
Sample: 0.1 % each, 10 μL
1.Erythromycin
2.Azithromycin
Column：Shodex Asahipak ODP-50 4D (4.6 mm I.D x 150 mm) Eluent：40 mM Potassium phosphate buffer (pH11.0)/CH3CN=40/60 Flow rate：0.5 mL/min Detector: UV (223nm) Column temp.：40 ℃
The reversed phase Asahipak ODP-40 4E column was used here to analyze simultaneously the macrolide antibiotic Erythromycin and its derivative Azithromycin. The polymer-based ODP-40 4E column is very alkaline-resistant and can therefore be used under alkaline conditions. *Sales of the product used in this application is discontinued, however if you are still interested in this application, please contact us. 
Sample : 10 μL 0.1 % each 1. Erythromycin 2. Azithromycin
Column : Shodex Asahipak ODP-40 4E (4.6 mm I.D. x 250 mm) Eluent : 40 mM Potassium phosphate buffer (pH11.0)/CH3CN=40/60 Flow rate : 0.5 mL/min Detector : UV (223 nm) Column temp. : 40 °C
