Cation ion-exchange chromatography column, Asahipak ES-502C 7C, was used to separate pochonicine and its analogues. Pochonicine is one type of polyhydroxylated pyrrolizidine alkaloids produced by filamentous fungi, Pochonia suchlasporia var. suchlasporia TAMA 87. The analogues have structures with one of the three hydroxides on the pyrrolizidine ring substituted with hydrogen. Analogue A and B are thought to have the hydrogen substituted on different positions.
Sample : 5µL
Fraction purified from the culture extract of
Pochonia suchlasporia var. suchlasporia TAMA 87
(Fungi culture provided by Dr. Toru Okuda, Tamagawa University Research Institute?
Pochonicine
Analogue A
Analogue B
Columns : Shodex Asahipak ES-502C 7C (7.5mmID*100mm)
Eluent?? : 10mM Ammonium bicarbonate aq.
Flow rate : 0.6mL/min
Detector : UV(210nm)
Column temp. : 40°C
Date provided by Dr. Teruhiko Nitoda, Faculty of Agriculture, Okayama University
