Refer to Instruments for On-Column Derivetization
Sample : 10micro-L
Histamine(Him) , Diethylenetriamine(Det:I.S.) , Tyramine(Tym) , 1,3-Diaminopropane(Dap)
Putrescine(Put) , Spermidine(Spd) , Spermine(Spm) , Cadaverine(Cad)
Column : Shodex Asahipak ODP-50 4D (4.6mmID*150mm)
Eluent : 50mM Borate buffer (pH9.9) - CH3CN(77:23,v/v)
containing 2mM OPA(o-Phthalaldehyde) and 2mM NAC(N-Acetyl-L-cysteine)
Flow rate : 0.5mL/min
Detector : Fluorescence detector(Ex 330nm,Em 430nm)
Column temp. : 40°C
K.Saito, M.Horie, N.Nose,K.Nakagomi and H.Nakazawa, Anal. Sci. 8,675(1992)
