Pyridylaminated (PA) monosaccharides were analysed by the Shodex SUGAR series
Asahipak NH2P-50 4E. Carbohydrates
are usually detected by refractive index detector (RI), but pyridylamination
of saccharides enables high sensitivity analysis by fluorescence detector.
Sample : 5µL
1. 2-Aminopyridine,
0.1pmol/µL
2. PA-Rhamnose,
1pmol/µL
3. PA-Fucose,
1pmol/µL
4. PA-Ribose,
1pmol/µL
5. PA-Xylose,
1pmol/µL
6. PA-N-Acetylglucosamine,
1pmol/µL
7. PA-N-Acetylgalactosamine,
1pmol/µL
8. PA-Mannose,
1pmol/µL
9. PA-Glucose,
1pmol/µL
10. PA-Galactose,
1pmol/µL
* Ratio of TPN [%] : TPN at a flow rate of 0.5mL/min is
set as 100%.
Column : Shodex Asahipak NH2P-50 4E (4.6mmID*250mm) Eluent : Phosphoric acid/H2O/CH3CN=1/14/85 Flow rate : 0.5mL/min Detector : Fluorescence (Ex. 310nm, Em. 380nm) Column temp. : 40°C