Analysis of pyridylaminated (PA) monosaccharides

Pyridylaminated (PA) monosaccharides were analysed by the Shodex SUGAR series
Asahipak NH2P-50 4E. Carbohydrates
are usually detected by refractive index detector (RI), but pyridylamination
of saccharides enables high sensitivity analysis by fluorescence detector.

Sample : 5µL

1. 2-Aminopyridine,
0.1pmol/µL

2. PA-Rhamnose,
1pmol/µL

3. PA-Fucose,
1pmol/µL

4. PA-Ribose,
1pmol/µL

5. PA-Xylose,
1pmol/µL

6. PA-N-Acetylglucosamine,
1pmol/µL

7. PA-N-Acetylgalactosamine,
1pmol/µL

8. PA-Mannose,
1pmol/µL

9. PA-Glucose,
1pmol/µL

10. PA-Galactose,
1pmol/µL

* Ratio of TPN [%] : TPN at a flow rate of 0.5mL/min is
set as 100%.

Column : Shodex Asahipak NH2P-50 4E (4.6mmID*250mm)
Eluent : Phosphoric acid/H2O/CH3CN=1/14/85
 Flow rate : 0.5mL/min
Detector : Fluorescence (Ex. 310nm, Em. 380nm)
Column temp. : 40°C