Analysis of Soft Drinks (LC/MS)

Canned soft drink containing coffee and sugar, tea and sugar and orange juice were analyzed using LC/MS. Stable baselines were obtained by using a polymer-based amino column, Asahipak NH2P-50 2D.

Sample : Soft drink
Canned coffee with sugar
1. Sucrose(m/z=378)

Canned tea with sugar
1. Sucrose(m/z=378)

Canned orange juice
1. Fructose(m/z=216)
2. Glucose(m/z=216)

 (HPLC)
 Instrument : Agilent 1100
 Column : Shodex Asahipak NH2P-50 2D (2.0mmID*150mm)
 Eluent : H2O/CH3CN=25/75
 Flow rate : 0.2mL/min
 Column temp. : 40°C
 (Post column)
 Reagent : CH3CN/CHCl3=50/50
 Flow rate : 0.2mL/min
 (MS)
 Instrument : Agilent 1100MSD
 Mass range : 100-500(m/z)
 Ionization : APCl
 Mode : SIM(M+Cl)
 Polarity : Negative
 Fragmentor : 20V
 Nebulizer : N2(40psi)
 Drying gas : N2(10L/min, 350°C)
 Corona current : 30micro-A
 Vaporizer temp. : 400°C