Analysis of Tribasic Organic Acids (SI-35 4D)
Please use this column with suppressor type ion chromatography system.
Citric acids and acnitic acids which are tribasic organic acids were analyzed using
IC SI-35 4D
(a column for anion analysis). In this analysis, citric acid and isocitric acid, cis-aconitic acid and trans-aconitic acid could be separated.
Sample : 20?L
1. Citric acid 10mg/L
2. Isocitric acid 50mg/L
3. trans-Aconitic acid 20mg/L
4. cis-Aconitic acid 20mg/L
Column : Shodex IC SI-35 4D (4.0mmI.D. x 150mm)
Eluent : 9.0mM Na2CO3 aq.
Flow rate : 0.6mL/min
Detector : Suppressed conductivity
Column temp. : 45°C
