Analysis of Tricarboxylic Acids (SI-35 4D)

Please use this column with suppressor type ion chromatography system.

Citric acids and aconitic acids which are tricarboxylic acids were analyzed using IC SI-35 4D (a column for anion analysis). In this analysis, citric acid and isocitric acid, cis-aconitic acid and trans-aconitic acid could be separated.

Sample : 20μL
1. Citric acid 10mg/L
2. Isocitric acid 50mg/L
3. trans-Aconitic acid 20mg/L
4. cis-Aconitic acid 20mg/L

Column       : Shodex IC SI-35 4D (4.0mmI.D. x 150mm)
Eluent       : 9.0mM Na2CO3 aq.
Flow rate    : 0.6mL/min
Detector     : Suppressed conductivity
Column temp. : 45°C