Analysis of Trisaccharides (NH2P-40 3E)

The trisaccharides melezitose, raffinose, and maltotriose were separated using Asahipak NH2P-40 3E, a polymer-based amino column.

Sample : 0.1% each, 10μL
1. Melezitose
2. Raffinose
3. Maltotriose

Column       : Shodex Asahipak NH2P-40 3E (3.0mmI.D. x 250mm)
Eluent       : CH3CN/H2O=75/25
Flow rate    : 0.35mL/min
Detector     : Shodex RI
Column temp. : 30℃