Analysis of Uric Acid and Creatinine using Column Switching Method

Uric acid and creatinine were analyzed using four columns Asahipak ES-502C, ES-502N, GS-310 7G and Asahipak GS-320 7C and column switching method. GS-310 7G is an old type of GF-310 HQ and GS-320 7C is an old type of GF-320 HQ.

Sample :
Cr: Creatinine
U: Uric acid

 Columns : C1 : Shodex Asahipak GS-320 7C (7.6mmID*100mm)
 C2 : Shodex Asahipak ES-502C 7C (7.6mmID*100mm)
 C3 : Dowex 50-X8
 C4 : Shodex Asahipak ES-502N 7C (7.6mmID*100mm)
 C5 : Shodex Asahipak GS-310 7G (7.6mmID*500mm)
 Eluent : 60mM Propionic acid + 15mM Succinic acid + 60mM NaOH in 1kg H2O (for C1, C2, C4, C5)
 50mM Propionic acid + 15mM Succinic acid + 60mM NaOH in 1kg H2O (for C3)
 Flow rate : 1.2mL/min
 Detector : UV(234nm,290nm)
 Column temp. : 30°C (for C1),40°C (for C2, C4, C5),45°C (for C3)

Courtesy of Dr. Seki, Osaka University