Bisphosphonates are the medicines used to treat osteoporosis by preventing bone absorption. In this application, the third generation zoledronic acid was analyzed using a non-suppressor type anion chromatography column, IC I-524A.
Sample: 100 µL
1. Zoledronic acid monohydrate 2 mg/mL
dissolved in H2O
Column : Shodex IC I-524A (4.6 mm I.D. x 100 mm) Eluent : 0.47 mL of Formic acid in 2500 mL of Water, pH3.5 (adjusted with 2 N NaOH aq.) Flow rate : 1.0 mL/min Detector : Shodex RI Column temp. : 35 °C