Analysis of Pyridylaminated Sugar Chains

Pyridylaminated (PA) sugar chains were separated using Asahipak NH2P-50 4E. Refractive Index detector (RI) is generally used for sugar analyses, however pyridylaminated sugar chains can be detected by more sensitive fluorescence detectors. Also the analysis can be carried under gradient elution.

Sample : 0.125pmol/μL, 5μL
1. PA-Sugar chain 012
2. PA-Sugar chain 013
3. PA-Sugar chain 014
4. PA-Sugar chain 001
5. PA-Sugar chain 015
6. PA-Sugar chain 002
7. PA-Sugar chain 010
8. PA-Sugar chain 004

Column       : Shodex Asahipak NH2P-50 4E (4.6mmI.D. x 250mm)
Eluent       : (A); 200mM Acetic acid-Triethylamine(pH7.3)/CH3CN=35/65
               (B); 200mM Acetic acid-Triethylamine(pH7.3)/CH3CN=50/50
               Linear gradient: 0 to 60min,  A to B
Flow rate    : 0.5mL/min
Detector     : Fluorescence(Ex: 310nm , Em: 380nm)
Column temp. : 40°C