According to USP 39 method, ribose should be analyzed using a column packed with L22. It is necessary for the system suitability to satisfy resolution of ribose/arabinose of ≥ 1.2, tailing factor of ≤ 1.5, theoretical plate number of ≥ 2,500 and relative standard deviation (RSD) of ≤ 2.0%. It was confirmed that the all conditions were satisfied when SUGAR KS-801 was used for analysis.
Sample : 10µL
1. Arabinose 0.02%
2. Ribose 2%
Column : Shodex SUGAR KS-801 (8.0mmI.D. x 300mm) Eluent : H2O Flow rate : 1.0mL/min Detector : Shodex RI Column temp. : 80°C
