According to the United States Pharmacopeia and the National Formulary (USP 40-NF 35*), the assay of tagatose should be used with a L22 column packed and meets the following requirements. The SUGAR SC1011 confirmed the requirements were met.
System suitability requirements:
Relative standard deviation (RSD): ≤ 2.0%
*The version at the time of the application acquisition.
Sample : 20µL
1. Tagatose 5mg/mL
Column : Shodex SUGAR SC1011 (8.0mmI.D. x 300mm) Eluent : 0.05mg/mL Calcium acetate aq. Flow rate : 0.6mL/min Detector : Shodex RI Column temp. : 85°C
