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Anion Standards (15) (Comparison of NI-424 and I-524A)


The seven main anions can be separated using a newly developed column, IC NI-424 quickly. The theoretical plate number of the column is more than double that of the existing column IC I-524A.
Comparison with I-524A column:
1) NI-424 column has achieved separation of phosphoric acid ion and fluoride ion which was difficult to sepatate using I-524A.
2) With NI-424, each peak is sharper, and separation of ions and peak balance have been improved. In particular, improvement has been made in the separation of chloride and nitrite ions making NI-424 columns suited to the analysis of a trace amount of nitrite ion contained in high-concentration chloride ion; demand for such analysis is high in the area of environmental analysis.

Sample : 20micro-L

1. H2PO4 10mg/L

2. F 1mg/L

3. Cl 1mg/L

4. NO2 5mg/L

5. Br 5mg/L

6. NO3 5mg/L

7. SO42- 5mg/L



(Left)
Column : Shodex IC I-524A (4.6mmID*100mm)
Eluent : 2.5mM Phthalic acid + 2.3mM Tris(hydroxymethyl)aminomethane aq.
Flow rate : 1.2mL/min
Detector : Shodex CD
Column temp. : 40°C

(Right)
Column : Shodex IC NI-424 (4.6mmID*100mm)
Eluent : 8mM 4-Hydroxybenzoic acid + 2.8mM
Bis-Tris + 2mM Phenylboronic acid + 0.005mM *CyDTA aq.

Flow rate : 1.0mL/min
Detector : Shodex CD
Column temp. : 40°C

* CyDTA: trans-1,2-Diaminocyclohexane-N,N,N’,N’-tetraacetic acid