Sample : 100micro-L
1. Cl–
2. NO2–
Column : Shodex IC NI-424 (4.6mmID*100mm)
Eluent : *18mM 4-Hydroxybenzoic acid + 2.8mM Bis-Tris
+ 2mM Phenylboronic acid + 0.005mM *2CyDTA(pH4.2)
Flow rate : 1.0mL/min
Detector : Shodex CD
Column temp. : 40°C
*1 The prescribed pH can be obtained by mixing the reagents in the composition described here.
*2 CyDTA: trans-1,2-Diaminocyclohexane-N,N,N’,N’-tetraacetic acid