The eluent used here is suitable for the simultaneous analysis of inorganic anions and dibasic and tribasic organic acids using IC I-524A. Organic acids are contained in beer and they interfere with the quantification of anions. Cl–
can be separated without such interference of organic acids. Organic acids, oxalic acid and citric acid also can be analyzed without interference.
Sample : 10-fold diluted Beer, 100micro-L
1. Acetic acid
2. H2PO4–, Succinic acid, Pyroglutamic acid
3. Lactic acid, Pyruvic acid
5. Malic acid, Br–
7. Oxalic acid
8. Citric acid
Column : Shodex IC I-524A (4.6mmID*100mm)
Eluent : 1.5mM Phthalic acid + 1.38mM Tris(hydroxymethyl)aminomethane
+ 300mM Boric acid aq.
Flow rate : 1.2mL/min
Detector : Shodex CD
Column temp. : 40°C