Anions in Beer

The eluent used here is suitable for the simultaneous analysis of inorganic anions and dibasic and tribasic organic acids using IC I-524A. Organic acids are contained in beer and they interfere with the quantification of anions. Cl^–, NO₃^– and SO₄^2- can be separated without such interference of organic acids. Organic acids, oxalic acid and citric acid also can be analyzed without interference.

Sample : 10-fold diluted Beer, 100micro-L

1. Acetic acid

2. H₂PO₄^–, Succinic acid, Pyroglutamic acid

3. Lactic acid, Pyruvic acid

4. Cl^–

5. Malic acid, Br^–

6. NO₃^–

7. Oxalic acid

8. Citric acid

9. SO₄^2-

 
 Column : Shodex IC I-524A (4.6mmID*100mm)
 Eluent : 1.5mM Phthalic acid + 1.38mM Tris(hydroxymethyl)aminomethane 
 + 300mM Boric acid aq.
 Flow rate : 1.2mL/min
 Detector : Shodex CD
 Column temp. : 40°C