Antifebriles (2) (DM-614)

Five components which are usually contained in the antifebriles were separated using RSpak DM-614 (a column for reversed phase chromatography).

Sample :
1. Aspirin (Acetylsalicylic acid)
2. Aminopyrine
3. Antipyrine
4. Caffeine
5. Phenacetin

 Column : Shodex RSpak DM-614 (6.0mmID*150mm)
 Eluent : 0.035M Na2HPO4 + 0.030M KH2PO4 + 35% CH3OH
 Flow rate : 0.6mL/min
 Detector : UV(254nm)
 Column temp. : 27°C