Saccharides and a sugar alcohol contained in apple juice were analyzed using SUGAR SC1011 (a column for saccharides analysis).
(Sample pretreatment)
1) Take 5g of apple juice in a beaker, add 30mL of pure water and neutralize with 10%(W/V) NaOH aq. cooled with ice.
2) Apply ultrasonic vibration for 30 minutes and make 50mL solution with pure water.
3) Filtrate with 0.45μm membrane filter.
Sample: Apple juice 5μL
1. Sucrose
2. Glucose
3. Fructose
4. Sorbitol.
Column : Shodex SUGAR SC1011 (8.0mmI.D. x 300mm) Eluent : H2O Flow rate : 0.6mL/min Detector : Shodex RI Column temp. : 85°C
