According to USP 40 method, the assay of polyethylene glycol 3350 should be analyzed using a column packed with L25. It is necessary for the system suitability to satisfy relative standard deviation (RSD) of ≤ 1.5%. It was confirmed that RSD was lower than 1.0% when polyethylene glycol 3350 was analyzed using OHpak SB-802.5 HQ.
Sample : 20μL
Polyethylene glycol 3350
20mg/mL in eluent
Column : Shodex OHpak SB-802.5 HQ (8.0mmI.D. x 300mm)
Eluent : 50 µg/mL of NaN3 aq.
Flow rate : 0.8mL/min
Detector : Shodex RI
Column temp. : 35°C
