Avidin, one of the saccaride-binding proteins, was analyzed using aqueous solvent SEC (GFC) column, PROTEIN
KW-804.
Sample : 50µL
1. Avidine
(Wako Pure Chemical Industries, Ltd.) 0.1%
Column : Shodex PROTEIN KW-804 (8.0mmID*300mm)
Eluent : 100mM Sodium phosphate buffer(pH7.0) + 0.3M NaCl
Flow rate : 1.0mL/min
Detector : UV(254nm)
Column temp. : 30° C