Barbiturates, which are widely used as hypnotics, have a maximum UV absorbance at 240nm when an alkaline eluent is used. These characteristics make it possible to obtain a highly sensitive analysis for barbiturates using Asahipak ODP-50 4D, a column for reversed-phase chromatography.
Sample :
1. Barbital
2. Phenobarbital
3. Hexobarbital
4. Pentobarbital
5. Secobarbital
Column : Shodex Asahipak ODP-50 4D (4.6mmI.D. x 150mm) Eluent : 50mM Phosphate buffer(pH11.0)/CH3OH=75/25 Flow rate : 0.5mL/min Detector : UV(254nm) Column temp. : 30°C
