Basic Peptides

Basic peptides were analyzed using Asahipak ODP-50 6D (a column for reversed phase chromatography).

Sample :
1. Sub.P Frg.1-4
2. Glu-Gly-Phe
3. Neuro.Frg.8-13
4. Met-Enkephalin
5. alpha-Endorphin
6. Des-Arg⁹-Bradykinin
7. Substance P

 Column : Shodex Asahipak ODP-50 6D (6.0mmID*150mm)
 Eluent : 0.05% TFA aq./CH3CN=80/20
 Flow rate : 1.0mL/min
 Detector : UV(220nm)
 Column temp. : 30°C