Benzaldehyde Inhibitors of Fermentation

Three kinds of benzaldehydes (4-hydroxybenzaldehyde, vanillin (vanilline (var.)) and syringaldehyde) known as inhibitors of fermentation derived from lignin were detected by UV and MS. The main two unknown peaks were detected at 58 minutes and 79 minutes on the UV Chromatogram. These components may be novel benzaldehydes. This method was applicable for analysis of the thermal hydrolysis sample and saccharification sample (The data of thermal hydrolysis sample is not shown here). Analysis time may be shortened by increasing the ratio of acetonitrile to water (see conditions below).

Sample : 20µL 1. 4-Hydroxybenzaldehyde 2. Vanillin 3. Syringaldehyde

Sample: 5μg/mL each（in Water）, 20μL
Instrument: Shimadzu Nexera/LCMS-8030
Column: RSpak GF-210 (7.0mmID*300mm)
Eluent: (A) Water/Formic acid=100/0.1(v/v)
Eluent: (B) Acetonitrile
Eluent: (Isocratic; (B%) 20%
Flow rate: 0.6 mL/min
Column temp.: 50℃
Detector: PDA & ESI-MS (SIM) + UV 280 nm

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