Benzalkonium chloride was analyzed using Asahipak GF-310 HQ ( a multisolvent GPC column ). Since benzalkonium chloride has benzene ring in the molecule, it can be detected by a UV detector better than by an RI detector.
Sample: 100ppm(UV), 0.1%(RI), 20µL
Benzalkonium chloride
Column : Shodex Asahipak GF-310 HQ (7.6mmID*300mm)
Eluent : CH3CN/50mM NaCl aq.=50/50
Flow rate : 0.6mL/min
Detector : UV(220nm), RI
Column temp. : 30°C