Benzoates were analyzed using RSpak DE-413 (a column for reversed phase chromatography).
Sample :
1. Methyl benzoate
2. Butyl benzoate
3. Hexyl benzoate
Column : Shodex RSpak DE-413 (4.6mmID*150mm)
Eluent : (left): CH3CN/H2O=65/35, (center): CH3CN/H2O=80/20, (right): THF/H2O=50/50
Flow rate : 0.6mL/min
Detector : UV(254nm)
Column temp. : 40°C