AFpak AAV-894 an affinity column has avidin (a protein in egg white) as a ligand. This column is recommended for the analysis of biotin derivatives.
Sample : Biotin 4-aminobenzoic acid
Column : Shodex AFpak AAV-894 (8.0mmID*50mm)
Eluent : (A); 0.01M Sodium acetate buffer(pH7.0) + 0.15M NaCl
(B); 0.1M Acetic acid/Isopropanol=50/50
Step gradient: (A) to (B)
Flow rate : 1.0mL/min
Detector : UV(260nm)
Column temp. : Room temp.
