AFpak ACB-894 (an affinity column) with a cibacron blue ligand is recommended for the analysis of albumin and NAD-dependent enzymes.
Sample : Bovine serum albumin
Column : Shodex AFpak ACB-894 (8.0mmID*50mm)
Eluent : (A); 0.1M Potassium phosphate buffer(pH5.0)
(B); 0.1M Potassium phosphate buffer(pH7.5) + 1.5M KCl
Step gradient: (A) to (B)
Flow rate : 1.0mL/min
Detector : UV(280nm)
Column temp. : Room temp.
