Saccharides are extracted from bread using water and three saccharides, lactose, glucose and fructose are separated using SUGAR KS-801 (a column for saccharides analysis). There is a unknown peak after the peak of fructose. It is recommended to use a guard column for the analysis of actual sample which may contain impurities.
Sample : Bread
1. Lactose
2. Glucose
3. Fructose
Columns : Shodex SUGAR KS-G 6B (6.0mmI.D. x 50mm) + KS-801 (8.0mmI.D. x 300mm) Eluent : H2O Flow rate : 1.0mL/min Detector : Shodex RI Column temp. : 80°C
