C-Reactive Protein

C-Reactive protein was analyzed using AFpak APE-894 (an affinity column).

Sample : C-Reactive protein (Sigma)

 Column : Shodex AFpak APE-894 (8.0mmID*50mm)
 Eluent : (A); Tris-HCl(pH7.0) + 0.15M NaCl + 1mM CaCl2 
 (B); (A) + 0.2M Phosphorylcholine
 Step gradient: (A) to (B) 
 Flow rate : 1.0mL/min
 Detector : UV(280nm)
 Column temp. : Room temp.