C-Reactive protein was analyzed using AFpak APE-894 (an affinity column).
Sample : C-Reactive protein (Sigma)
Column : Shodex AFpak APE-894 (8.0mmID*50mm)
Eluent : (A); Tris-HCl(pH7.0) + 0.15M NaCl + 1mM CaCl2
(B); (A) + 0.2M Phosphorylcholine
Step gradient: (A) to (B)
Flow rate : 1.0mL/min
Detector : UV(280nm)
Column temp. : Room temp.
