Typical saccharides were analyzed using SUGAR SZ5532 and calibaration curves for the saccharides were obtained. Every calibaration curves passes the origin and has good linearity. All the corelation coefficients for the saccharides are greater than 0.9999. The calibration curves were obtained from peak heights. They can be also obtaind from the peak area satisfactorily.
Sample : 10micro-L
Coefficient of determination
(1 to 200μg)
Column : Shodex SUGAR SZ5532 (6.0mmID*150mm) Eluent : CH3CN/H2O=75/25 Flow rate : 1.0mL/min Detector : Shodex RI Column temp. : 60°C