Carbohydrates of fructooligosaccharides were analyzed using SUGAR KS-802 and KS-801 . Connecting two columns with different exclusion limits can facilitate the separation only using water as eluent.
Sample : 15mg/L each, 20µL
1-Fructofuranosyl-D-nystose
Nystose
1-Kestose
Sucrose
Glucose
Fructose
Column : Shodex SUGAR KS-802 + KS-801 (8.0mmID*300mm each) Eluent : H2O Flow rate : 0.5mL/min Detector : Shodex RI Column temp. : 85° C