Carcinogenic tryptophan pyrolysis products were analyzed using Asahipak ES-502C 7C (a column for weak cation exchange chromatography).
Column : Shodex Asahipak ES-502C 7C (7.6mmID*100mm)
Eluent : 20mM H3PO4(pH2.0)/CH3CN=90/10
Flow rate : 1.0mL/min
Detector : Fluorescence detector(Ex. 266nm, Em. 397nm)
Column temp. : 40°C
S Manabe and O. Wada, Exp. Eye Res., 48 (1989) 351.